Preliminary Genetic Analysis of the Sugarcane Population Aa40

Materials and methods The AA40 population has shown good segregation for DNA Extraction various ecoiomically important characteristics. -AS such, SASEX is accumulating both molecular and phenotypic data DNA was extracted from N18 and CP571614 and the 150 on this cross in an attempt to generate a genomic map and progeny of AA40 using a methodology modified from discover DNA markers linked to measurable Del'aporta et ('983). Leaf tissue (3-6 g) was frozen in liquid nitrogen and ground to a fine powder before being DNA from a of AA40 Progeny and transferred to a sterile 50 m] tube containing 35 m] of the two putative parents were cut with two restriction extraction buffer mM Tris-HC1 (pH 8,jj , 500 m~ the purified and blotted On memNaCI, 50 mM EDTA (pH 8,O) and 1% (v/v) P-rnercaptobranes. These were then probed with two sugarcane genomic ethanol). Tubes were shaken vigorously, after which 3,5 ml DNA sequences which had been radioactively labeled. of 20 % (wlv) SDS was added. Sam~le s were incubated at From the large number of shared bands on these RFLP blots, it is evident that the AA40 progeny arose from a single crossing event. At the same time, there are sufficient polymorphisms between the individuals to make the cross suitable for molecular mapping. RFLP results also showed that there were bands present in the progeny which were not present in either of the two parents, NI8 or CP571614. On closer examination it became clear that the male parent of the cross had been misidentified. Experiments are currently under way in an attempt to identify this parent.